We tested handwashing techniques with a simple experiment in our Microbiology Lab at Hamilton General Hospital. Rinse your hands well under clean, running water. apply soap and lather/scrub every nook and cranny of your hands for 20-30 seconds (about the time to sing "Happy Birthday" twice). sections: following Purpose you the experiment. Student 2 proceeds to wash for one minute the thumb of the right hand under a stream of warm running water using a scrubbing motion between the thumb and the right index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the R2 section of the “Water” plate using a zigzag motion. good laboratory practices and technique, (2) safety equipment, and (3) facility design. This laboratory exercise illustrates the critical need for proper hand washing techniques as a means of reducing the incidence of healthcare facility acquired infections.  The exercise compares the effectiveness of two hand washing techniques: using water only, and using soap and water.  The student is exposed to the fact that the hands harbor microorganisms that could result in transmitting disease.  In addition, the microorganisms resident in the hands could result in contamination of laboratory experiments if aseptic techniques, such as wearing gloves, are not strictly followed. Transient flora are the microorganisms you pick up from what you have been handling. Needed for Each Lab To participate in the lab, you must have the following items: 1. The three main elements of safe containment of microorganisms are (1) good laboratory practices and technique, (2) safety equipment, and (3) facility design. Analysis of total lab automation instruments finds limited risk from processing specimens, but the lab environment faces exposure from staff’s handling of samples, especially without strict adherence to safety protocols. . This experiment asks them to test antimicrobial chemicals for use in soaps. Rinse and dry thoroughly. The number of colonies were counted by visual inspection of the plates.  The percent of microorganism growth was calculated as: percent reduction = [colonies (section 1) – colonies (section x)] – colonies (section 1), where x = plate sections 2, 3, 4 for each hand.  Table 1.2 show the results of the calculations.  The percent reduction for the right hand shows a linear rate.  The percent reduction for the left hand shows initially an increase and then a linear rate of decline. Most are naturally occurring and are harmless, but some may be disease-causing germs. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. Student 2 proceeds to wash for 2 minutes the thumb of the right hand under as stream of warm running water using a scrubbing motion between the thumb and the right index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the R3 section of the “Water” plate using a zigzag motion. Hand Washing Video by CDC Start studying Hand washing - Microbiology. This preview shows page 1 - 2 out of 4 pages. Vigorous hand washing… Course Hero is not sponsored or endorsed by any college or university. This experiment will basically test whether bacteria are better cleaned using soap, drying with paper towels, air drying, or using chemicals like hydrogen peroxide or alcohol Dry your hands using a clean towel or air dry them; Note: Use soap or alcohol-based hand sanitizer to get rid of … Date: SEP.17.2020 // … Figure 1.2 Agar plates inoculated with microorganisms from the left hand thumb. All plates are to be incubated in an inverted position at 37 degrees Celsius for a period of 48 hours. 1 Hand washing is a simple, convenient, effective, and cost-effective means to limit the transmission of communicable diseases from one person to another. 5 April 2014. HAND HYGIENE 1 2. Residential flora is difficult to remove with simple hand washing.  Transient flora is easier to remove.  Surgical scrubbing is the best way to remove transient flora and reduce the presence of residential flora. . 3. Student 2 proceeds to add a drop of antibacterial soap to the pad of his left hand thumb and then wash for 3 minutes the thumb under a stream of warm running water using a scrubbing motion between the thumb and the left index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the L4 section of the “Soap” plate using a zigzag motion. 2. Your hands should be wet throughout the washing. I (Lauren) joined the team just before the … Washington, DC – September 18, 2019 – Rubbing hands with ethanol-based sanitizers should provide a formidable defense against infection from flu viruses, which can thrive and spread in saliva and mucus. Cdc.gov. Thoroughly washing hands is the single most important thing students can do to keep from getting sick, or to keep from infecting others. According to research literature, hand washing with water only is ineffective.  Using soap for extended periods of time is a better alternative to using only water.  However, this allows some transient flora to cling deeply to hair and to remain protected by oil and dead skin cells.  Soap with surgical scrubbing is a good way to remove transient flora and some residential flora. ehp Handwashing 101 LESSON: Summary: Students develop a hypothesis about the most effective handwashing method, develop an experiment protocol to test their hypothesis, and carry out their handwashing experiment. Web. Your lab coat (will be provided to you by Valencia)-must be … Purpose: The purpose of this experiment is to test the effectiveness of washing and drying hands on the number of bacteria on the hands. How does hand washing affect residential versus transient flora? Washing hands without soap is much less effective. C. EFFECTIVENESS OF HAND WASHING . Hand Washing Lab Report - Hand Washing Effectiveness In Cold Vs Hot Water Adele Weston Introduction Hand washing is an important method of prevention, 21 out of 25 people found this document helpful, Hand Washing Effectiveness In Cold Vs. Print. Here is an excellent example of how to write an unknown lab report in Microbiology class. Take two sterile …
At the end of … This is my video submission for the Hand Washing Lab for my Microbiology class. Student 1 uses aseptic technique to soak a sterile cotton swab in sterile saline solution in a test tube.  Student 1 then proceeds to rub the moistened cotton swab on the pad of the left hand thumb of Student 2 and then inoculates the L1 section of the “Soap” plate using a zigzag pattern from the edge of the dish towards the dividing middle line. Microbiology studies microscopic spoilage organisms such as bacteria, viruses and pathogens. Correct hand-washing is the single most effective technique that prevents the transmission of micro-organisms either directly or indirectly to others. . All healthcare providers ultimately have to take a microbiology class as part of their program curriculum. Two hand-washing methods, using hot and cold water with soap, were tested through colonial growth observation to determine how successful they were in the removal of bacteria. Lather your hands both front and back including the fingers and nails, by rubbing them together with the soap. Lesson Type: Experiment and Extension Lesson—Students collect, manipulate, and/or summarize data from … Proper hand washing can protect you and others from a range of diseases. Click to see … Applying a large volume of hand sanitizer ensures excess active ingredient and … Microbiology laboratories should be designed and have sufficient space to suit the operations … RePort Laboratory Fall2008 MicrobiologY handwashins/fomites the data from the interpreting and report summarizing Write a into the It should be divided your wellwritten. Cappuccino, James, and Natalie Sherman. Microbiology: A Laboratory Manual. . It's important to have control plates to distinguish between bacteria that possibly came from the substance/object you were using and the resident/transient bacteria on the hand. Hand Washing: Weak Link in Preventing Microbial Contamination in Labs. 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